R&D Systems™ Recombinant Human CNTF Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Product Code	Brand	Quantity	Formulation
18178520	R&D Systems™ 257-NT-010	10 μg	Lyophilized from a 0.2μm filtered solution in PBS, NaCl, EDTA, DTT and CHAPS with BSA as a carrier protein.
18187690	R&D Systems™ 257-NT-050	50 μg	Lyophilized from a 0.2μm filtered solution in PBS, NaCl, EDTA, DTT and CHAPS with BSA as a carrier protein.
18334837	R&D Systems™ 257-NT-010/CF	10 μg	Lyophilized from a 0.2μm filtered solution in PBS, NaCl, EDTA, DTT and CHAPS.
18146870	R&D Systems™ 257-NT-050/CF	50 μg	Lyophilized from a 0.2μm filtered solution in PBS, NaCl, EDTA, DTT and CHAPS.

Description

The Recombinant Human CNTF Protein is derived from E. coli. The Recombinant Human CNTF Protein has been validated for the following applications: Bioactivity.

Specifications

• Gene ID (Entrez): 1270
• Name: CNTF
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 3 months, 2 to 8° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.1 EU per 1μg of the protein by the LAL method.
• Recombinant: Recombinant
• Purity or Quality Grade: 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Molecular Weight (g/mol): 22.8 kDa
• Source: E. coli-derived human CNTF protein Ala2-Met200
• Structural Form: This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2 - 22.8 kDa.
• Conjugate: Unconjugated
• Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.03-0.18 μg/mL. The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR ) .