R&D Systems™ Recombinant Human Nectin-4 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Brand:  R&D Systems™ 2659-N4-050

Product Code. 18158260

Description

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 x 104 cells/well are added to recombinant human Nectin-4 coated plates (10 μg/mL with 100 μL/well), approximately >45% will adhere after 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: NP_112178
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 44 kDa, reducing conditions; M.W. (theoretical): 35.6 kDa
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Protein: Nectin-4/PVRL4

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 81607
• Name: Nectin-4
• Source: Mouse myeloma cell line, NS0-derived human Nectin-4 protein Gly27-Val351, with a C-terminal 6-His tag
• Structural Form: Monomer
• Gene Symbol: NECTIN4
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.