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R&D Systems™ Recombinant Human Ubiquitin Activating Enzyme (UBE1), CF
Recombinant Protein
Brand: R&D Systems™ E-305-025
This item is not returnable.
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Description
Recombinant Human Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.Specifications
P22314.3 | |
Supplied as a solution in HEPES, NaCl and TCEP. | |
MolecularWeight-theroretical: 118 kDa | |
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein | |
A1S9T, A1S9T and BN75 temperature sensitivity complementing, A1ST, AMCX1, GXP1, MGC4781, POC20, POC20 centriolar protein homolog, Protein A1S9, SMAX2, UBA1, UBA1, ubiquitin-activating enzyme E1 homolog A, UBA1A, UBE1, UBE1A1S9, UBE1X, Ubiquitinactivating Enzyme, Ubiquitin-activating enzyme E1, ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing), ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier-activating enzyme 1 | |
Unconjugated | |
Ubiquitin-activating Enzyme/UBE1 |
Enzyme Activity | |
7317 | |
25 μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening. | |
UBA1 | |
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain. |
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