R&D Systems™ Recombinant Mouse Tryptase beta-1/Mcpt7 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Brand:  R&D Systems™ 1937-SE-020

Product Code. 18321328

Description

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >75 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q02844
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
• Molecular Weight (g/mol): M.W. (Observed): 36 kDa and 37 kDa, reducing conditions; M.W. (theoretical): 28.8 kDa
• Quantity: 20 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 17230
• Name: Tryptase beta-1/MCP-7/Mcpt7
• Source: Mouse myeloma cell line, NS0-derived mouse Tryptase beta-1/MCP-7 protein Ile29-Phe273, with an C-terminal 10-His tag
• Structural Form: Mature form
• Gene Symbol: Tpsab1
• Recombinant: Recombinant
• Protein: Tryptase beta-1/MCP-7/Mcpt7