R&D Systems™ Recombinant Mouse VSIG4 Fc Chimera Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Brand:  R&D Systems™ 4674-VS-050

Product Code. 18166550

Description

Measured by its ability to inhibit anti-CD3-induced proliferation of splenic T lymphocytes. Vogt, L. et al. (2006) J. Clin. Invest. 116:2817. The presence of immobilized Recombinant Mouse VSIG4 Fc Chimera inhibited the anti-CD3 response by =50%. The ED50 for this effect is 1.25-5 μg/mL.

Specifications

• Accession Number: NP_808457
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 60-65 kDa, reducing conditions; M.W. (theoretical): 46 kDa (monomer)
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Protein: VSIG4

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 11326
• Name: VSIG4 Fc Chimera
• Source: Mouse myeloma cell line, NS0-derived mouse VSIG4 protein Mouse VSIG4 (His20-Pro187) (N-terminus) Accession # NP_808457 IEGRMDP Mouse IgG2A (Glu98-Lys330) (C-terminus)
• Structural Form: Disulfide-linked homodimer
• Gene Symbol: Vsig4
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.