BD Biosciences™ Regulatory T-Cell Function Kit (RUO/GMP)

Measures expression of the activation markers CD154 and/or CD69 on effector T cells.

Brand:  BD Biosciences™ 648956

Product Code. 15876319

Description

• Naturally occurring human CD4⁺ regulatory T cells (Tregs) are critical mediators of self-tolerance and suppress aberrant or inappropriate immune responses that can lead to conditions such as type 1 diabetes, rheumatoid arthritis, and graft-vs-host disease.
• The BD FastImmune™ kit measures expression of the activation markers CD154 and/or CD69 on effector T cells.
• Reduced expression of these markers in the presence of Tregs can indicate Treg suppressive capacity.
• The assay is performed in 96-well microplates using peripheral blood mononuclear cells (PBMCs) activated in the presence and absence of Tregs.
• It is a short-term assay that can be set up and analyzed in approximately one day.
• The T-cell markers, CD3 and CD4, are included in the kit for T-cell gating purposes, and CD25 is included for defining the Treg population.
• Contents: Regulatory T-Cell function cocktail (CD3 PerCP-Cy™5.5, CD4 FITC, CD25 PE); CD69 PE-Cy™7; CD154 APC; EDTA.

Specifications

• For Use With (Equipment): Flow cytometer
• Includes: CD154, APC, CD4 FITC/CD25 PE/CD3 PerCP-CY 5.5, CD69, PE-CY7, EDTA
• Regulatory Status: RUO/GMP

• Formulation: CD154, APC, CD4 FITC/CD25 PE/CD3 PerCP-CY 5.5, CD69, PE-CY7, EDTA
• Quantity: 50 Tests
• Content And Storage: Store reagents at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light.