Thermo Scientific™ Gram Stain Kit

Differentiate gram-positive organisms from gram-negative organisms using Gram Stain Kit.

Brand:  Thermo Scientific™ R40080

Product Code. 11953492

Description

Differentiate gram-positive organisms from gram-negative organisms using Thermo Scientific™ Remel™ Gram Stain Kit. The Gram stain method was developed in 1884 by the Danish bacteriologist, Christian Gram, to differentiate bacterial cells from infected tissue¹. Later it was discovered that the bacterial cell wall composition was the key to the Gram stain and would differentiate bacteria into two groups based on cell color after staining. Since that time there have been many modifications of the original technique^2,3.

Use of gram staining kit helps in differentiating gram-positive organisms from gram-negative organisms.

• Ready to use: Liquid reagents supplied in 250mL bottles
• Ease of identification: As it stains the gram positive organisms as purple and gram-negative organisms as red
• Rapid results: 5-10 mins for the staining

The gram staining kit consists of 4 bottles containing 250 mL of Gram Crystal Violet, Safranin, Gram Iodine and Gram Decolorizer each.

Crystal Violet, a basic dye, is the primary stain taken up by all bacteria due to its ability to rapidly permeate the cell wall. It stains the protoplast of bacteria purple. The potassium-iodine mixture, in gram iodine, is the mordant which complexes with the primary stain in the cell. In gram-positive cells, the crystal violet-iodine complex is trapped in the cell due to a decrease in cell wall permeability caused by alcohol dehydration. Gram-positive cells are stained purple.

In gram-negative cells, the complex is removed by the Decolorizer due to an increase in permeability caused by solubility of the lipids in alcohol.

The counterstain used must be a contrasting color to the primary stain and in this case it is Safranin, which stains gram-negative cells red.

Not all products are available for sale in all territories. Please inquire.

Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

• Type: Gram Stain Kit
• Quantity: 4/kit
• Includes: Crystal Violet; Decolorizer; Iodine; Safranin

• Volume (Metric): 4 x 250 mL
• Certifications/Compliance: CE Marked

Safety and Handling

Product Identifier

• Gram Stain Kit

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 2
• Flammable liquid Category 3
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H226-Flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H336-May cause drowsiness or dizziness.
• H412-Harmful to aquatic life with long lasting effects.
• EUH066-Repeated exposure may cause skin dryness or cracking.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P273-Avoid release to the environment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P501-Dispose of contents/container to .

Supplemental information

• MIXTURE LIST-Contains : Iodine, Ethyl alcohol, Methyl alcohol, Safranin O, Ethyl alcohol, Methyl alcohol, Phenol, C.I. Basic