Applied Biosystems™ RNA UltraSense™ One-Step Quantitative RT-PCR System

RNA UltraSense™ One-Step Quantitative RT-PCR System

Brand:  Applied Biosystems™ 11732927

Product Code. 10616743

Description

The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript™ III RT and Platinum™ Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/μl total RNA can be detected with a PCR efficiency of 97% (Figure 1). The RNA UltraSense™ System features:

SuperScript™ III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure

Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity

Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes

Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure

Validated performance on multiple real-time instrument platforms

One-step format for speed, convenience, and less reaction-to-reaction variability

Applications:
Real-time quantitative amplification of RNA, including:

RNA viruses

Low-abundance RNA transcripts

Difficult-to-amplify RNA targets

High-value RNA detection and research

Specifications

• Content And Storage: The RNA UltraSense™ One-Step Quantitative RT-PCR System is supplied with the following components: 250 μl RNA UltraSense™ Enzyme Mix (includes SuperScript™ III RT, Platinum™ Taq DNA Polymerase, and RNaseOUT™ Ribonuclease Inhibitor); 2 x 1 ml RNA UltraSense™ 5X Reaction Mix; 300 μl 20X Bovine Serum Albumin (BSA), Ultrapure Molecular Biology Grade (5 mg/ml); 1 ml 50-mM magnesium sulfate (MgSO₄ ); 100 μl ROX Reference Dye. Sufficient reagents are provided for 100 reactions. Store components at -20°C. Stability can be extended by storing at -80°C. ROX Reference Dye must be stored in the dark.
• GC-Rich PCR Performance: High
• Polymerase: DNA Polymerase
• Product Line: Platinum™, SuperScript™, UltraSense™
• Quantity: 100 rxns
• Sufficient For: 100 Reactions
• Sample Type: RNA

• Detection Method: Primer-probe
• PCR Method: 1-step RT-qPCR
• Reaction Speed: Standard
• Product Type: One-Step Quantitative RT-PCR System
• Shipping Condition: Dry Ice
• No. of Reactions: 100 Reactions

For Research Use Only. Not for use in diagnostic procedures.