Gibco™ RPMI 1640 Medium

RPMI 1640 Medium

Product Code	Brand	With Additives	Without Additives	Quantity
11530586	Gibco™ 21875034	Glutamine, Phenol Red	No HEPES, No Sodium Pyruvate	500 mL
11540586	Gibco™ 21875042	Glutamine, Phenol Red	No HEPES, No Sodium Pyruvate	100 mL
10432512	Gibco™ 21875059	Glutamine, Phenol Red	No HEPES, No Sodium Pyruvate	1000 mL
11560586	Gibco™ 21875109	Glutamine, Phenol Red	No HEPES, No Sodium Pyruvate	20 x 100 mL
10247302	Gibco™ 21875158	Glutamine, Phenol Red	No HEPES, No Sodium Pyruvate	6 x 1000 mL
11554446	Gibco™ 31870082	Phenol Red	No Glutamine, No HEPES, No Sodium Pyruvate	20 x 100 mL

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications.



Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ RPMI 1640 product made in our Grand Island facility (11875-127). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Product Type: RPMI 1640 Medium
• Regulatory Status: For in-vitro diagnostic use

• Form: Liquid
• For Use With (Application): Mammalian cell culture

Safety and Handling

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