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Gibco™ RPMI 1640 Medium (Dutch modification) (1X)
RPMI 1640 Medium (Dutch modification) (1X)
Brand: Gibco™ 22409031
This item is not returnable.
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Description
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. This RPMI formulation has the Dutch modification (addition of HEPES, with sodium bicarbonate lowered to 1 g/L).
Product Intended Use
For Research Use Only. Not for use in diagnostic procedures.
cGMP Manufacturing and Quality System
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (1.0 g/L) and therefore requires a 5-7% CO2 environment to maintain physiological pH.
Specifications
ISO 13485 standards, FDA | |
RPMI 1640 Medium, 500mL, 1 X Concentration, Liquid Form, With Phenol Red, HEPES, 12 Months Shelf Life, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture | |
Liquid | |
Phenol Red, HEPES | |
Mammalian cell culture | |
For research use only |
2 to 8°C | |
1 X | |
RPMI 1640 Medium | |
No Glutamine, No Sodium Pyruvate | |
10 x 500 mL |
For Research Use Only. Not for use in diagnostic procedures.