Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement

RPMI 1640 Medium, GlutaMAX™ Supplement

Brand:  Gibco™ 61870044

Product Code. 12027599

Description

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Using RPMI 1640 Medium
RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Product intended use
For in vitro diagnostic use. CAUTION: not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
RPMI 1640 is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical RPMI 1640 product made in our Grand Island facility (61870-036). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standards.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Description: Roswell Park Memorial Institute (RPMI) 1640 Medium, 10 x 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With Glutamax™, Phenol Red, 12 Months Shelf Life, 260 to 310mOsm/kg Osmolality, For Mammalian Cell Culture
• Form: Liquid
• With Additives: GlutaMAX, Phenol Red
• For Use With (Application): Mammalian cell culture
• Quantity: 10 x 500mL

• Content And Storage: 2 to 8°C
• Concentration: 1 X
• Product Type: Roswell Park Memorial Institute (RPMI) 1640 Medium
• Without Additives: No HEPES, No Sodium Pyruvate
• pH: 7.0 to 7.4