Gibco™ RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

RPMI 1640 Medium, GlutaMAX™ Supplement, HEPES

Product Code	Brand	Quantity
11534526	Gibco™ 72400013	100 mL
11544526	Gibco™ 72400021	500mL
11564526	Gibco™ 72400112	20 x 100mL
11554526	Gibco™ 72400054	10 x 500mL

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂ and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. Gibco™ RPMI 1640 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

Dual-Site cGMP Manufacturing
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ RPMI 1640 product made in our Grand Island facility (72400-047). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Product Type: Roswell Park Memorial Institute (RPMI) 1640 Medium
• Without Additives: No Sodium Pyruvate

• Form: Liquid
• With Additives: GlutaMAX, Phenol Red, HEPES
• For Use With (Application): Mammalian cell culture