Gibco™ RPMI 1640 Medium, HEPES, no glutamine

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Product Code	Brand	Quantity
11554496	Gibco™ 42401018	500 mL
11564496	Gibco™ 42401042	10 x 500 mL

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:
With: Phenol Red, HEPES
Without: L-glutamine

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Concentration: 1 X
• Form: Liquid
• Without Additives: No Glutamine, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard

Safety and Handling

missing translation for 'shelfLife' : 12 Months

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.