Gibco™ RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Brand:  Gibco™ 42402016

Product Code. 11574496

Description

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI 1640 is modified as follows:

• With: Phenol Red, HEPES
• Without: L-glutamine

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Use

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Culture Environment: CO₂
• Classification: Animal Origin-free
• Culture Type: Mammalian Cell Culture
• Product Type: RPMI 1640
• Sterility: Sterile-filtered
• Without Additives: No Glutamine, No Sodium Pyruvate
• Green Features: Sustainable packaging
• Osmolality: 270–300 mOsm/kg
• Product Line: Gibco™
• Shipping Condition: Room Temperature

• Content And Storage: Storage: 2°C to 8° C, protect from light
  Shipping: Ambient
  Shelf life: 12 months from date of manufacture
• Description: Roswell Park Memorial Institute (RPMI) 1640 Medium, 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With Phenol Red, HEPES, 12 Months Shelf Life, 270 to 300mOsm/kg Osmolality, For Mammalian Cell Culture
• Concentration: 1X
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: HEPES, Phenol Red, Sodium Bicarbonate
• For Use With (Application): Mammalian cell culture
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• pH: 7 to 7.4
• Quantity: 500 mL

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.