STEMCELL Technologies RSeT™ Feeder-Free Medium

Serum-free medium for naïve-like human pluripotent stem cells

Brand:  STEMCELL Technologies 05975

Product Code. 16467933

Description

• Revert primed human embryonic (ES) and induced pluripotent stem (iPS) cells to a naïve-like state and maintain cells in a naïve-like state under hypoxic conditions without bFGF or feeder cells
• Developed under license from the Weizmann Institute of Science, this serum-free cell culture medium produces robust cultures with features of a naïve-like state, such as tightly packed, domed colonies with refractive edges
• Human ES/iPS cells cultured in RSeT Feeder-Free Medium show increased expression of key transcripts associated with the naïve-like state, such as KLF17, KLF2, KLF4, and TFCP2L1, and can either be differentiated or converted back to a primed state by culture in mTeSR1 and then differentiated
• Compatible products that can be used for differentiation include STEMdiff Definitive Endoderm Kit (Catalog #05110), STEMdiff SMADi Neural Induction Kit (Catalog #08581), and STEMdiff Mesoderm Induction Medium (Catalog #05220)

Specifications

• Cell Type: Pluripotent Stem Cells
• For Use With (Application): Cell Culture, Expansion, Maintenance
• Quantity: 500 mL Kit