Thermo Scientific Chemicals Safranine O

Product Code	Brand	Quantity
11349729	Thermo Scientific Chemicals B21674.09	10 g
11359729	Thermo Scientific Chemicals B21674.18	50 g

Description

It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage.

Solubility
Soluble in water at 20°C, 50g/L. Soluble in alcohol.

Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Chemical Identifiers

• CAS: 477-73-6
• Molecular Weight (g/mol): 350.85
• InChI Key: QRYAEWIQIBAZOJ-UHFFFAOYSA-N
• PubChem CID: 2723800
• SMILES: [Cl-].CC1=C(N)C=C2C(=C1)N=C1C(C)=C(N)C=CC1=[N+]2C1=CC=CC=C1

• Molecular Formula: C20H19ClN4
• MDL Number: MFCD00011759
• Synonym: basic red 2, safranine o, gossypimine, safranin, safranine t, safranin o, safranin t, safranine, tolusafranine, hidaco safranine
• IUPAC Name: 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride

Specifications

• CAS: 477-73-6
• Molecular Formula: C20H19ClN4
• Beilstein: 3924099
• Solubility Information: Soluble in water at 20°C,50g/L. Soluble in alcohol.
• SMILES: [Cl-].CC1=C(N)C=C2C(=C1)N=C1C(C)=C(N)C=CC1=[N+]2C1=CC=CC=C1
• Molecular Weight (g/mol): 350.85
• Formula Weight: 350.85

• Odor: Odorless
• MDL Number: MFCD00011759
• Synonym: basic red 2, safranine o, gossypimine, safranin, safranine t, safranin o, safranin t, safranine, tolusafranine, hidaco safranine
• InChI Key: QRYAEWIQIBAZOJ-UHFFFAOYSA-N
• IUPAC Name: 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine;chloride
• PubChem CID: 2723800
• Chemical Name or Material: Safranine O

Safety and Handling

GHS H Statement
H315-H319-H335
Causes skin irritation.
Causes serious eye irritation.
May cause respiratory irritation.

P264b-P280-P302+P352-P305+P351+P338-P310-P332+P313-P362

H315-H318

missing translation for 'einecsNumber' : 207-518-8

missing translation for 'rtecsNumber' : SG1623000

missing translation for 'tsca' : Yes

Recommended Storage : Ambient temperatures

Product Identifier

• Safranine O

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : Safranine O

RUO – Research Use Only