Invitrogen™ Second Strand cDNA Synthesis Kit

Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Brand:  Invitrogen™ A48570

Product Code. 17195802

Description

InvitrogenTM Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Features of the Second Strand cDNA Synthesis Kit include:

• Efficient synthesis of full-length double-stranded cDNA
• Fast—procedure completed in 1 hour
• Convenient—pre-mixed components
• Complete—includes residual RNA removal reagents

Applications

• Full-length double-stranded blunt-end cDNA synthesis for cloning
• Double-stranded cDNA library construction
  

Specifications

• Content And Storage: Contents:
  • Second Strand Enzyme Mix, 1 mL
  • 5X Second Strand Reaction Mix, 250 μL
  • 0.5 M EDTA, pH 8.0, 1 mL
  • RNase I, 10 U/μL, 500 μL
  • Water, nuclease-free, 3 x 1.25 mL
  
  Storage: -15 to -25°C
  
  
• Product Type: cDNA Synthesis Kit
• Final Product Type: Double Stranded cDNA
• Reaction Format: Separate Components

• Format: 50 reactions
• For Use With (Application): Cloning
• No. of Reactions: 50 Reactions