Cytiva Sepharose™ Fast Flow Cation Exchange Media

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Product Code	Brand	Binding Capacity	Product Type	Packaging Quantity
10576350	Cytiva 17-0729-10	70 mg Ribonuclease A/mL mediμm	SP Sepharose Fast Flow Media	25 mL
10724592	Cytiva 17-0729-01	70 mg Ribonuclease A/mL mediμm	SP Sepharose Fast Flow Media	300 mL
10714592	Cytiva 17-0719-01	50 mg Ribonuclease A/mL mediμm	CM Sepharose Fast Flow Media	500 mL
10059859	Cytiva 17-0719-10	50 mg ribonuclease A/mL mediμm	CM Sepharose Fast Flow Media	25 mL

Description

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

 

• Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
• Composed of crosslinked 6% agarose beads
• Reproducible
• Part of Sepharose Fast Flow ion exchange platform
• High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
• Available in range of pre-packed process development tools

 

SP Sepharose:

• Ligand: Sulphopropyl
• Ion Exchanger Type: Strong cation exchanger
• Ionic Capacity: 0.18 to 0.25mmol H⁺/mL medium
• Binding capacity: 70mg ribonuclease A/mL medium

CM Sepharose:

• Ligand: Carboxymethyl
• Ion Exchanger Type: Weak cation exchanger
• Ionic Capacity: 0.09 to 0.13mmol Hl⁺/mL medium
• Binding capacity: 50mg ribonuclease A/mL medium

 

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

Specifications

• Particle Size: 45 to 165 μm
• pH Range: 4 to 13

Safety and Handling

Product Identifier

• SP Sepharose Fast Flow, 25 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-18 per cent of the mixture consists of component(s) of unknown acute toxicity.