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Cytiva Sera-Xtracta Virus/Pathogen Kit
For efficient, total nucleic acid (DNA/RNA) isolation from bacteria and viruses including COVID-19
Brand: Cytiva 29506009
Description
- Sera-Xtracta Virus/Pathogen Kit for high-throughput total nucleic acid (DNA/RNA) isolation from bacteria and viruses including Adenovirus (Type 14), Influenza A (H3N2) and COVID-19.
- Offers the advantages of solid phase extraction (using magnetic beads) and reproducible yields.
- Simplified protocol can be adapted for both manual and automated high-throughput processing.
- Scalable up to 400 μL of sample.
- Rapid extraction procedure can be completed in less than 30 minutes.
- For use with respiratory biological matrices, blood and universal transport media to support the sensitive downstream detection of viruses and other pathogens found in low concentrations.
- Compatible with molecular biology techniques, including quantitative polymerase chain reaction (qPCR, RT-qPCR), droplet digital PCR (ddPCR), and next-generation sequencing (NGS).
- Sera-Xtracta Virus/Pathogen Kit provides a simple and rapid method to optimize the workflow for sensitive detection system for viruses and other pathogens found in low concentrations.
- Optimize isolation workflows to reduce bottlenecks
- To address this bottleneck, our robust extraction chemistry ensures that the total nucleic acid is selectively bound to the superparamagnetic beads, while impurities are efficiently removed during the quick wash steps.
- The resulting high quality total nucleic acid is then eluted from the bead using a suitable elution buffer of choice.
- The rapid Sera-Xtracta workflow gives pure, high-quality nucleic acid suitable for sensitive molecular testing.
Infectious diseases affect millions of people every year. Particularly virulent and multi-drug resistant agents are increasingly responsible for infections with ever-expanding complexities. The design, manufacture and validation of assays for these pathogenic agents requires the successful purification of high-quality nucleic acid. This is essential to any molecular research and testing workflow. The nucleic acid purification process can be a bottleneck because sufficient nucleic acid from biological samples is required to meet a sensitivity threshold for assays which are designed to help identify, map or make informed decisions on latent and active infections.
Specifications
All reagents can be stored at ambient temperature (15°C–25°C). For Proteinase K, once reconstituted, store at 2°C–8°C | |
96 Purifications | |
Can be completed in less than 30 minutes |
Binding/Lysis Reagent 60 mL; Wash Buffer 100 mL; SeraSil-Mag 400 beads 1.1 mL; SeraSil-Mag 700 beads 1.1 mL; Proteinase K 30 mg (lyophilized) | |
Up to 400 μL of sample |
Safety and Handling
- Sera-Xtracta Virus/Pathogen Kit (96 extractions)
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335-May cause respiratory irritation.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : thiocyanic acid, hydrochloric acid, proteases
- UNKNOWN ACUTE-20 per cent of the mixture consists of component(s) of unknown acute toxicity.