IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.

Product Code	Brand	Protein Tag	Vector
15603888	IBA Lifesciences 5-5214-001	His Tag (6x), Strep-tag II	pDSG-IBA45
15613888	IBA Lifesciences 5-5219-001	Twin-Strep-tag	pDSG-IBA102
15623888	IBA Lifesciences 5-5220-001	Twin-Strep-tag	pDSG-IBA103
15633888	IBA Lifesciences 5-5221-001	Twin-Strep-tag	pDSG-IBA104
15643888	IBA Lifesciences 5-5222-001	Twin-Strep-tag	pDSG-IBA105
15663878	IBA Lifesciences 5-5200-001	Untagged	pDSG-IBAwt1
15673878	IBA Lifesciences 5-5201-001	Untagged	pDSG-IBAwt2
15693878	IBA Lifesciences 5-5211-001	His Tag (6x), Strep-tag II	pDSG-IBA43

Description

pDSG-IBA vectors for MEXi mammalian expression system

• Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
• Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
  • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
  • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
  • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

• Constitutive or Inducible System: Constitutive
• Promoter: CMV
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Species: Mammalian
• Concentration: 250 ng/μL

• Marker Gene: Ampicillin
• Product Type: Vector
• Content And Storage: Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Cloning Method: Direct Cloning
• Quantity: 5 μg