IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

Product Code	Brand	Description	Protein Tag	Includes
15653778	IBA Lifesciences 5-4400-001	pESG-IBA wt1	Untagged	Ampicillin Resistance cassette and Neomycin resistance gene
15663778	IBA Lifesciences 5-4401-001	pESG-IBA wt2	Untagged	BM40 signal
15673778	IBA Lifesciences 5-4403-001	pESG-IBA 3	Strep-tag for C-terminal	Neomycin resistance gene
15683778	IBA Lifesciences 5-4405-001	pESG-IBA 5	Strep-tag for N-terminal	Neomycin resistance gene
15693778	IBA Lifesciences 5-4423-001	pESG-IBA 23	Strep-tag for C-terminal and GST-tag for N-terminal	Neomycin resistance gene
15603788	IBA Lifesciences 5-4425-001	pESG-IBA 25	GST Tag for N-terminal	Neomycin resistance gene
15613788	IBA Lifesciences 5-4433-001	pESG-IBA 33	His Tag (6x) for C-terminal	Neomycin resistance gene
15623788	IBA Lifesciences 5-4435-001	pESG-IBA 35	His Tag (6x) for N-terminal	Neomycin resistance gene
15633788	IBA Lifesciences 5-4443-001	pESG-IBA 43	His Tag (6x) for N-terminal and Strep-tag for C-terminal	Neomycin resistance gene
15643788	IBA Lifesciences 5-4445-001	pESG-IBA 45	Strep-tag for N-terminal and His Tag (6x) for C-terminal	Neomycin resistance gene
15653788	IBA Lifesciences 5-4462-001	pESG-IBA 62	Strep-tag for C-terminal and FLAG-tag for N-terminal	Neomycin resistance gene
15663788	IBA Lifesciences 5-4463-001	pESG-IBA 63	FLAG-tag for C-terminal	Neomycin resistance gene
15673788	IBA Lifesciences 5-4464-001	pESG-IBA 64	Strep-tag for N-terminal and FLAG-tag for C-terminal	Neomycin resistance gene
15683788	IBA Lifesciences 5-4465-001	pESG-IBA 65	FLAG-tag for N-terminal	Neomycin resistance gene
15693788	IBA Lifesciences 5-4502-001	pESG-IBA 102	Twin-Strep-tag for C-terminal	BM40 signal, Ampicillin Resistance cassette and Neomycin resistance gene
15603798	IBA Lifesciences 5-4503-001	pESG-IBA 103	Twin-Strep-tag for C-terminal	Neomycin resistance gene
15613798	IBA Lifesciences 5-4504-001	pESG-IBA 104	Twin-Strep-tag for N-terminal	BM40 signal, Ampicillin Resistance cassette and Neomycin resistance gene
15623798	IBA Lifesciences 5-4505-001	pESG-IBA 105	Twin-Strep-tag for N-terminal	Neomycin resistance gene
15633798	IBA Lifesciences 5-4523-001	pESG-IBA 123	Twin-Strep-tag for C-terminal and GST-tag for N-terminal	Neomycin resistance gene
15643798	IBA Lifesciences 5-4542-001	pESG-IBA 142	His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal	BM40 signal and Neomycin resistance gene
15653798	IBA Lifesciences 5-4543-001	pESG-IBA 143	His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal	Neomycin resistance gene
15663798	IBA Lifesciences 5-4545-001	pESG-IBA 145	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal	Neomycin resistance gene
15673798	IBA Lifesciences 5-4562-001	pESG-IBA 162	Twin-Strep-tag for C-terminal and FLAG-tag for N-terminal	Neomycin resistance gene
15683798	IBA Lifesciences 5-4564-001	pESG-IBA 164	Twin-Strep-tag for N-terminal and FLAG-tag for C-terminal	Neomycin resistance gene
15693798	IBA Lifesciences 5-4567-001	pESG-IBA 167	Twin-Strep-tag/FLAG-tag for N-terminal	Neomycin resistance gene
15603808	IBA Lifesciences 5-4568-001	pESG-IBA 168	Twin-Strep-tag/FLAG-tag for C-terminal	Neomycin resistance gene

Description

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

• NeoR for generation of stable cells lines.
• Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
• SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
• ColE1 + Amp: for propagation in E. coli
• NeoR: neomycin resistance gene to generate stable cell lines
• BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

• Format: Liquid
• Inducing Agent: SV40 large T antigen (e.g. COS1 or COS7)
• Promoter: Human CMV Immediate-early
• Storage Buffer: 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
• Concentration: 250 ng/μL
• Color: Clear

• For Use With (Equipment): StarGate™ System, Mammalian Host Cells
• pH: 8
• Product Type: Acceptor Vector for mammalian expression
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Quantity: 5 μg
• For Use With (Application): Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

Safety and Handling

missing translation for 'shelfLife' : 12 months