IBA Lifesciences StarGate pYSG-IBA Vectors for Yeast Expression

Designed for high-level expression of recombinant proteins in Yeast by introducing the gene of interest under the control of the Cu++-inducible CUP1 promoter.

Marke:  IBA Lifesciences 5-4625-001

Artikelnummer. 15653808

Beschreibung

pYSG-IBA yeast expression vectors

The pYSG-IBA vector allows the high-level expression of proteins in yeast.

• The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
• The expressed recombinant protein will be localized in the cytoplasm.

 

• High-level expression in yeast with high copy number
• Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
• Tightly regulated expression due to Cu⁺⁺-inducible CUP1 promoter
• Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
• Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
• URA3 auxotrophy marker for selection after transformation

Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Spezifikation

• Format: Liquid
• Description: pYSG-IBA 25
• Marker Gene: leu2-d and URA3
• Promoter: CUP1
• Storage Buffer: 20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
• Protein Tag: GST Tag for N-terminal
• Quantity: 5 μg
• For Use With (Application): Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

• For Use With (Equipment): StarGate™ System, Yeast Host Cells
• Inducing Agent: Copper sulfate
• pH: 8
• Product Type: Acceptor Vector for yeast expression
• Content And Storage: 2°C to 8°C for frequent usage, -20°C for long-term storage
• Concentration: 250 ng/μL
• Color: Clear