Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Product Code	Brand	Kit Contents	Quantity
10445345	Thermo Scientific™ 34075	Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL	100 mL
10220294	Thermo Scientific™ 34076	Luminol/Enhancer, 100mL, Stable Peroxide Buffer, 100mL	200 mL
10398773	Thermo Scientific™ 37071	Stable Peroxide Buffer, 10mL, Luminol/Enhancer, 10mL	20 mL

Description

Thermo Scientific™ SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems and quantitation. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, enabling multiple film or camera exposures.

SuperSignal West Dura Extended Duration Substrate characteristics include:

• Sensitivity: mid-femtogram
• Stability: 24-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 24 hours
• Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Specifications

• Product Type: Substrate
• Form: Liquid
• Substrate: Horseradish Peroxidase
• Signal Duration: 24 hr.

• Detection Method: Chemiluminescence
• Product Line: SuperSignal™
• Sensitivity: Mid-femtogram

For Research Use Only. Not for use in diagnostic procedures.