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  5. Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
  6. 15659364

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Brand:  Thermo Scientific™ 34577

62.22 GBP valid until 2024-12-31
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Product Code. 15659364

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Description

Description

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:

  • Sensitivity: pictogram to femtogram sensitivity
  • Stability: 8-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 6 to 24 hours
  • Recommended primary antibody concentration: 1:1000–1:5000 dilution (0.2–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Substrate
Liquid
Nitrocellulose, PVDF
200 mL
Horseradish Peroxidase
Picogram to high femtogram-level
25 Mini-Gel Blots, 2000 cm2 of Membrane
Sufficient for 25 mini-gel blots; 2000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL
• SuperSignal West Pico PLUS Stable Peroxide, 100 mL

Store at room temperature.
Chemiluminescence
Western Blot
SuperSignal™
HRP
2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
6 to 24 hr.
SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide
Room Temperature
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