SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Pico PLUS

This substrate kit is comprised of SuperSignal West Femto Maximum Sensitivity Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both high sensitivity and routine western blotting.

Brand:  Thermo Scientific A43841

Product Code. 17153209

Description

SuperSignal West Femto Maximum Sensitivity Substrate

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult to detect targets.

• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: 8-hr working solution stability, 1-year kit stability at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to 8 hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

SuperSignal West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is best for routine, everyday western blotting applications, providing excellent signal intensity, sensitivity and duration across a wide range of targets.

• Sensitivity: pictogram to femtogram sensitivity
• Stability: 8-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

Specifications

• Product Type: Substrate
• Form: Liquid
• Membrane Compatibility: Nitrocellulose, PVDF
• Quantity: 1 Bundle
• Sensitivity: Picogram to femtogram, low femtogram (mid-zeptomole)
• Content And Storage: • 200 mL SuperSgnal West Femto reagent, store at 2–8°C
  • 20 mL SuperSignal West Pico PLUS reagent, store at room temperature
  
  

• Detection Method: Chemiluminescence
• For Use With (Application): Western Blot
• Product Line: SuperSignal™
• Substrate: Horseradish Peroxidase
• Sufficient For: 60 Mini-Gel Blots, 5000 sq. cm of membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
• Shipping Condition: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.