Thermo Scientific™ Taq DNA Polymerase, native, without BSA (5 U/μL)

Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.

Brand:  Thermo Scientific™ EP0282

Product Code. 10829230

Description

Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.

Highlights

• Thermostable—half life is more than 40 min at 95°C.
• Generates PCR products with 3'-dA overhangs.
• Supplied with two buffers—10X Taq Buffer with KCl and 10X Taq Buffer with (NH₄)₂SO₄. The latter allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.
• Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).

Applications

• Routine PCR amplification of DNA fragments up to 5 kb
• DNA labeling (see Reference s 1-3)
• High throughput PCR

Specifications

• Content And Storage: • Taq DNA Polymerase, native (5 U/μL), 100 μL
  • 10X Taq Buffer with KCl, 2 x 1.25 mL
  • 10X Taq Buffer with (NH₄)₂SO₄, 2 x 1.25 mL
  • 25 mM MgCl₂, 2 x 1.25 mL
  
  Store at -20°C.
• Polymerase: Taq DNA Polymerase
• Product Type: Taq DNA Polymerase
• For Use With (Application): Standard PCR
• Fidelity (vs. Taq): 1X
• Overhang: 3'-A
• Starting Material: DNA

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Quantity: 500 Units
• Concentration: 5 U/μL
• Hot Start: No
• Reaction Format: Separate Components
• Volume: 2 mL

For Research Use Only. Not for use in diagnostic procedures.