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TaqMan® TaqMan™ Gene Expression Assay, VIC
Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5' end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3' end.
£272.00 - £1012.00
Specifications
Detection Method | Primer-probe |
---|---|
Format | Tube |
GC-Rich PCR Performance | High |
PCR Method | qPCR |
Reaction Speed | Fast |
Alert:
This Product Requires Additional Configuration
Please order Applied Biosystems™ TaqMan™ Gene Expression Assays through the Gene Expression Search Tool.
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
11927021
|
TaqMan®
4448489 |
S (360 reactions), made to order |
£272.00
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
11957021
|
TaqMan®
4448490 |
M (750 reactions), made to order |
£493.00
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
11947021
|
TaqMan®
4448491 |
L (2900 reactions), made to order |
£1012.00
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
Applied Biosystems TaqMan Gene Expression assays, VIC, are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end.
Features include:
• Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
• Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
• Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
• Accurate—identify small fold-changes with high accuracy of quantitation
• Extensive content—over 1.8 million predesigned assays available for over 25 different species
• Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
• Ideal for multiplexing—combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well
Approximate ship time
Made to order: 4–6 days in North America, 6–10 days in Europe
Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration)
TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents. All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.*
Recommended master mix:TaqMan Fast Advanced Master Mix (Cat. No. 4444556)
*Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.
Specifications
Primer-probe | |
High | |
Fast | |
Sustainable packaging | |
60 X |
Tube | |
qPCR | |
7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex | |
Gene Expression Assay |
For Research Use Only. Not for use in diagnostic procedures.
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