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Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

Brand: Applied Biosystems™ 4488593

2360.11 GBP valid until 2024-12-14
Use promo code "21632" to get your promotional price.

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Product Code. 15344674

£3540.00 / 200 reactions

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Alert:

To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21632” to get your promotional price

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Description

TaqMan™ PreAmp Master Mix lets you: • Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded tissues (FFPE) • Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays • Analyze up to 100 gene expression targets with minimal hands-on time Preserves Equilibrium of Targets Without Preamplification Bias: In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA. Easy Workflow: With TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp™ 9700 thermal cycler. For Research Use Only. Not for use in diagnostic procedures.

Order Info

Guaranteed minimum shelf life is 60 days.

Specifications

Content And Storage	Contains one 5-mL bottle of 2X master mix. Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format	Bottle
PCR Method	qPCR
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Type	PreAmp Master Mix
For Use With (Application)	Pre-amplification
Sample Type	cDNA

Detection Method	Primer-probe
GC-Rich PCR Performance	High
Reaction Speed	Standard
Product Line	TaqMan™
Sufficient For	200 Reactions
No. of Reactions	200 Reactions
Volume	5 mL

Product Suggestions

For Research Use Only. Not for use in diagnostic procedures.