Applied Biosystems™ TaqMan™ Protein Expression Core Reagents Kit

TaqMan™ Protein Expression Core Reagents Kit

Brand:  Applied Biosystems™ 4405501

Product Code. 10489594

Description

Applied Biosystems™ TaqMan™ Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan™ Protein Expression Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan™ protein expression assays. The Universal PCR Assay contains optimized concentrations of TaqMan™ probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems™ real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan™ Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan™ Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry. Key features of the TaqMan™ Protein Expression Assay:

Quantitates protein expression using gold standard TaqMan™ 5’ nuclease chemistry

Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform

Allows greater sensitivity and a more quantitative readout than western blots and immunostaining.

Specifications

• Content And Storage: Includes the TaqMan™ protein expression core reagents base kit (with DNA ligase, ligase dilution buffer, ligation reaction buffer, PBS pH 7.4, protease, and universal PCR assay; store at –15 to –25°C), and the TaqMan™ protein expression fast master mix (store at 2 to 8°C)
• For Use With (Equipment): 7500 Fast system, 7900HT fast system, StepOne™, fast mode, StepOnePlus™, fast mode
• Product Type: TaqMan™ Protein Expression Core Reagents Kit
• Quantity: 100 rxn(s)
• For Use With (Application): Protein Expression Analysis, Stem Cell Analysis
• No. of Reactions: 100 rxn(s)

• Detection Method: Primer-probe
• Label or Dye: FAM
• Purity or Quality Grade: UP (Ultra Pure)
• Target: Not target-specific
• Final Product Type: TaqMan™ Protein Expression Core Reagents kit
• Sample Type: Cells (general)

For Research Use Only. Not for use in diagnostic procedures.