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TCR V beta 5.1/5.2 Monoclonal Antibody (MR9-4), APC, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 17-5796-80
Description
Description: This MR9-4 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 5.1/5.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR9-4 antibody recognizes the V beta 5.1 and 5.2 chains on T cells from mouse strains with the b haplotype of the Tcrb gene, including C57BL/6, B10, and C58/J. V beta 5.1 and 5.2 are deleted in mouse strains that express MHC Class II I-E, including Balb/c, C3H, and DBA/2. Reports also indicate that V beta 5+ T cells recognize the OVA peptide. Applications Reported: This MR9-4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MR9-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
TCR V beta 5.1/5.2 Monoclonal antibody specifically detects TCR V beta 5.1/5.2 in Mouse samples. It is validated for Flow Cytometry.Specifications
TCR V beta 5.1/5.2 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
Mouse | |
25 μg | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
MR9-4 | |
APC | |
TCR V beta5.1; TCR V beta5.2; TCRV beta 5.1; TCRV beta 5.2; TCRV beta5.1; TCRV beta5.2; Vb5.1; Vb5.2; Vbeta5.1; Vbeta5.2 | |
Affinity chromatography | |
RUO | |
Mouse | |
Antibody | |
IgG1 κ |
For Research Use Only.