Thermo Scientific™ FastDigest MnlI (IIs class)

The FastDigest MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

Brand:  Thermo Scientific™ FD1074

Product Code. 10561031

Includes: Two versions of the universal FastDigest buffer are supplied with each enzyme: 10X FastDigest Buffer and 10X FastDigest Green Buffer. All FastDigest enzymes are 100% active in both buffers. Enzymes used in common downstream applications such as ligation, blunting and dephosphorylation also have 100% activity in both buffers.

Description

The FastDigest MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features
• 100% activity of all FastDigest enzymes in the universal buffer
• 100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Direct loading on gels
• No star activity
• 176 FastDigest enzymes available

Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

Specifications

• Content And Storage: • FastDigest MnlI (50 μL)
  • 10X FastDigest Buffer (1.0 mL)
  • 10X FastDigest Green Buffer (1.0 mL)
  
  Store at –20°C.
  
  
• Enzyme: MnlI
• Sensitive to Heat Inactivation: Yes
• Type IIS RE: Yes
• Product Type: Restriction Enzyme
• Product Line: FastDigest™
• Inactivation Temperature: 65°C

• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Compatible Buffer: 10X FastDigest Buffer/FastDigest Green Buffer
• Optimal Reaction Temperature: 37°C
• Quantity: 50 μL (50 Reactions)
• Research Category: Scarless Cloning
• Overhang: 3' protruding ends

For Research Use Only. Not for use in diagnostic procedures.