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Thermo Scientific™ GeneJET Endo-Free Plasmid Maxiprep Kit
The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column.
Brand: Thermo Scientific™ K0861
156.11 GBP valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).
Description
The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights:
- Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
- High concentration of DNA purified—> 400 ng/μL
- Convenient—spin column format, centrifuge or vacuum protocols
- Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
- Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- Transfection of robust cell lines
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription
Includes
- Resuspension Solution
- Lysis Solution
- Neutralization Solution
- Endotoxin Binding Reagent
- Wash Solution I (concentrated)
- Wash Solution II (concentrated)
- RNase A Solution
- Elution Buffer (10 mM Tris-HCl, pH 8.5)
- GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL)
- Collection tubes (50 mL)
Hazardous: No
Quality Control:
- The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E. coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual.
- The kit passes QC requirements if the purified plasmid DNA has an A260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis.
- The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases.
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
- Transfection of robust cell lines
- FastDigest restriction enzyme digestion
- Automated fluorescent sequencing
- PCR
- Cloning
- Transformation
- In vitro transcription
Order Info
Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.
Specifications
400 ng/μL | |
Spin Column | |
Maxi | |
Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR | |
10 Preps | |
GeneJET | |
10 Preps | |
60 min. |
Spin Column | |
Silica Column | |
Bacteria | |
Not High-throughput Compatible (Manual) | |
> 200 μg (Large-Scale) Plasmid DNA | |
Endo-Free Plasmid MaxiPrep Kit | |
Plasmid DNA |
Safety and Handling
- GeneJET Endo-Free Plasmid Maxiprep Kit
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Guanidine hydrochloride, acetic acid, sodium lauryl sulphate, sodium hydroxide
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For Research Use Only. Not for use in diagnostic procedures.