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Thermo Scientific™ GeneJET Endo-Free Plasmid Maxiprep Kit

The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column.

Brand:  Thermo Scientific™ K0861

156.11 GBP valid until 2024-12-31
Use promo code "21651" to get your promotional price.



Product Code. 15236187

  • £233.00 / 0.46kg

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Includes: Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).
This item is not returnable. View return policy

Description

Description

The Thermo Scientific GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights:

  • Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
  • High concentration of DNA purified—> 400 ng/μL
  • Convenient—spin column format, centrifuge or vacuum protocols
  • Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

  • Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
  • Transfection of robust cell lines
  • FastDigest restriction enzyme digestion
  • Automated fluorescent sequencing
  • PCR
  • Cloning
  • Transformation
  • In vitro transcription

Includes

  • Resuspension Solution
  • Lysis Solution
  • Neutralization Solution
  • Endotoxin Binding Reagent
  • Wash Solution I (concentrated)
  • Wash Solution II (concentrated)
  • RNase A Solution
  • Elution Buffer (10 mM Tris-HCl, pH 8.5)
  • GeneJET Maxi Purification Columns pre-assembled with collection tubes (50 mL)
  • Collection tubes (50 mL)

Hazardous: No

Quality Control:

  • The GeneJET Plasmid Maxiprep Kit is qualified by isolating high copy number plasmid DNA from 250 mL of overnight E. coli culture (OD600 = 2) grown in LB medium following the protocol outlined in the manual.
  • The kit passes QC requirements if the purified plasmid DNA has an A260/280 ratio between 1.8 and 1.9 and a dominant band of supercoiled plasmid DNA is observed after agarose gel electrophoresis.
  • The functional quality of the plasmid DNA is evaluated by digestion with restriction endonucleases.

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

  • Transfection of robust cell lines
  • FastDigest restriction enzyme digestion
  • Automated fluorescent sequencing
  • PCR
  • Cloning
  • Transformation
  • In vitro transcription

Order Info

Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

400 ng/μL
Spin Column
Maxi
Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
10 Preps
GeneJET
10 Preps
60 min.
Spin Column
Silica Column
Bacteria
Not High-throughput Compatible (Manual)
> 200 μg (Large-Scale) Plasmid DNA
Endo-Free Plasmid MaxiPrep Kit
Plasmid DNA
Product Suggestions

Product Suggestions

Videos
Safety and Handling

Safety and Handling

Product Identifier
  • GeneJET Endo-Free Plasmid Maxiprep Kit

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.

Precautionary Statement
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Guanidine hydrochloride, acetic acid, sodium lauryl sulphate, sodium hydroxide

SDS
Documents

Documents

Certificates
Special Offers

Special Offers

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