Phalloidin Control, DyLight 554, Invitrogen™

Control

Brand:  Invitrogen 21834

Product Code. 13218118

Description

Phalloidin Control, DyLight 554 conjugate, 21834, from Invitrogen., 21834, from Invitrogen. Species Reactivity: Many; Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry.

Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom and is commonly used as a counterstain (similar to DAPI or Hoechst) in cell biology and histology imaging applications to selectively label F-actin in fixed cells, permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates have similar affinity for both large and small filaments and bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells. Phalloidins reportedly do not bind to monomeric G-actin, unlike some antibodies against actin. The dynamics of the actin polymerization in cells are important for a variety of cellular processes from cell motility to cell shape, from muscular contraction to cytokinesis, and more.
Cross Reactivity: Many Species
Formulation: 300 units purified control antibody (300 units/ml) in methanol and no preservative
Synonyms: DY554-Phalloidin, F-actin toxin, Amanita phalloides
Format: 300 units of lyophilized DyLight 554-Phalloidin (300 units/ml) in methanol. Prepare stock solution by adding 1000 µL of pure methanol to the vial and gently mix. 21834 has been successfully used in immunofluorescence and immunohistochemistry. DyLight 554-Phalloidin has an excitation/emission of 551/572nm and molecular weight of 1313.5 g/mole. DyLight 554-Phalloidin Stock Solution can be prepared in methanol, DMF, or DMSO and unused Stock Solution should be promptly stored at -20°C to minimize evaporation. Working solutions (per 96-well plate) can be prepared by diluting 20 µL of the Stock Solution in 5.98mL of PBS and mixing well. Typical staining procedure adds 50µl of Working Solution (i.e. 1 to 5 unit/ml) to a each well. Incubate cells in the dark for 30 minutes at room temperature (optimal staining times varies from 10 minutes to 3 hours depending on cell type). Aspirate and wash cells three times in PBS after incubation. If desired, probe with specific antibodies or dyes before using DyLight 554-Phalloidin.

Specifications

• Antigen: Phalloidin
• Concentration: 300 units/mL
• Gene Alias: DY554-Phalloidin, F-actin toxin, Amanita phalloides
• Quantity: 300 U
• Primary or Secondary: Isotype Control (Primary)
• Content And Storage: -20°C

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry
• Conjugate: DyLight 554
• Purification Method: purified
• Regulatory Status: RUO
• Target Species: Multi-species
• Form: Lyophilized