Invitrogen™ TIMP2 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHTIMP2X5

Product Code. 17135579

Description

The Human Tissue Inhibitor of MetalloProteinases 2 (TIMP-2) ELISA quantitates Hu TIMP-2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TIMP-2. Principle of the method The Human TIMP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Specifications

• Accession Number: P16035
• Assay Sensitivity: 2 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 7077
• Interassay CV: <12%
• Kit Contents: Biotinylated Detection Antibody, Adhesive Plate Covers, Standard, Wash Buffer, Stop Solution, Assay Diluent Concentrate, Chromogen, SAV-HRP, Pre-coated 96 well plates
• Quantity: 5 x 96 Tests
• Research Discipline: Other Research
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 1.6 pg/mL to 1200 pg/mL
• Calibration Range: 25 pg/mL to 7000 pg/mL
• Conjugate: Biotin
• Protein Family: Proteases, Peptidases & Inhibitors
• Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL- 8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-gamma, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, PDGF, SCF, TNF-alpha, TNF-beta, TPO, VEGF.
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CSC-21 K,DDC8
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 μ;L; Serum, 1 μ;L; Supernatant, 100 μ;L
• Synonym: CSC-21 K, DDC8
• Test Time: 1 hr. 20 min.