Invitrogen™ Total Nitric Oxide Assay Kit

Measure total nitric oxide (NO) as the single value (sum) of nitrate (NO3-) and nitrite (NO2-) levels in serum, plasma, urine, saliva and other biological fluids.

Brand:  Invitrogen™ EMSNOTOT

Product Code. 10762345

Description

Assay total nitric oxide (NO) as the single value (sum) of nitrate (NO₃^-) and nitrite (NO₂^-) levels in urine, saliva, plasma, serum and other biological fluids with our Thermo Scientific Total Nitric Oxide Assay Kit.

Additional Information and Online Ordering

Nitric oxide assay kit is for quantitative determination of nitrite (NO₂^-) and nitrate (NO₃^-) in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540nm. Total nitric oxide (NO) contributed by nitrate and nitrite in a system is measured as nitrite after converting all nitrate to nitrite.

• Target: Total nitric oxide (NO) contributed by the sum of nitrite (NO₂^-) and nitrate (NO₃^-)

• Format: Colorimetric stain (azo dye) for clear 96-well plates

• Measurement: Absorbance at 540nm (±20nm)

• Assay Range: 3.1 to 100μM

• Sensitivity: 1.35μM

• Precision: Dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)

• Sample Types: Urine, saliva, plasma, serum and other biological fluids

• Total Assay Time: 45 minutes

• Sample Size: 50μL

Includes:Complete kit contains Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.

Specifications

• No. of Tests: 2 x 96 tests

For Research Use Only. Not for use in diagnostic procedures.