Phasemaker™ TRIzol™ Reagent and Phasemaker™ Tubes Complete System

This system includes both TRIzol™ Reagent and Invitrogen™ Phasemaker™ Tubes.

Product Code	Brand	Quantity
15609415	Phasemaker™ A33250	100 Preps
15608948	Phasemaker™ A33251	200 Preps

Description

This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes. The TRIzol Reagent offers superior lysis capability, even with difficult sample types. Invitrogen™ Phasemaker Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol mix from the organic phase underneath, making very easy to pipette off the upper phase containing the RNA.

The polymer works on density gradient, as it is heavier than the aqueous phase, but lighter that the organic phase; after centrifugation it positions itself between these two layers. It creates a tight seal between the two phases, making it very easy to pipette off the aqueous phase without the risk of carrying over contaminants from the organic layer. Because this, Invitrogen Phasemaker Tubes allow you to recover the whole aqueous phase, increasing yield up to 30%. It is an inert polymer that does not interfere with downstream experiments such as PCR. It works with fresh or frozen samples (tissue or blood). It also works with tissue samples treated previously with RNAlater. Invitrogen Phasemaker Tubes come already pre-dispensed. The tube size is 2 mL.

• Complete System includes TRIzol and Phasemaker Tubes
• Phasemaker Tubes are pre-dispensed in 2 mL tubes
• Creates a tight seal between the aqueous and the organic phases
• Easier to pipette off the whole aqueous phase
• Increases yield up to 30%
• RNA integrity is not compromised
• The seal stays for longer periods of time, allowing to increase the number of samples per batch
• Inert, does not interfere with downstream experiments

Warranty

One year after receiving

Specifications

• Isolation Technology: Organic Extraction
• Elution Volume: 20 to 50 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
• Starting Material Amount: Bacteria: ≤10⁷ cells
  Cells: ≤10⁷
  Plant: ≤10⁷ cells, 100 mg
  Tissue: ≤100 mg
  Yeast: ≤10⁷ cells

• Purification Time: 1 hr.
• Sample Type: Bacteria, Cells, Plants, Tissue, Yeast
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Shipping Condition: Room Temperature
• Yield: Cells: ≤15 μg
  Tissue: ≤73 μg

For Research Use Only. Not for use in diagnostic procedures.