Invitrogen™ TrueCut™ HiFi Cas9 Protein (5 μg/μL)

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

Product Code	Brand	Quantity
17494673	Invitrogen™ A50577	500 μg

Description

• A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency
• Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
• Maintains maximum on-target editing efficiency compared to TrueCut™ Cas9 Protein v2
• Transfection-ready using either electroporation or lipid-mediated transfection reagents
• Manufactured under strict ISO-13485 quality standards
• Ideal for applications that are sensitive to off-target events and when more precise editing is required
• Eliminates time consuming cloning steps

Available in Two Concentrations

• 1 μg/μL - For use in standard editing scenarios
• 5 μg/μL - For optimized editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
• For custom sizes or concentrations, please inquire

Specifications

• Concentration: 5 μg/μL
• Sensitive to Heat Inactivation: Yes
• Product Type: HiFi Cas9

• Purity: ≥90%
• Source: E. coli
• Form: Liquid