Gibco™ TrypLE™ Select Enzyme (10X), no phenol red

TrypLE™ Select Enzyme (10X), no phenol red

Brand:  Gibco™ A1217701

Product Code. 10272625

Description

TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:

• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols, with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective

This TrypLE™ Select is modified as follows:

With
• EDTA

Without
• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.

Specifications

• Chelators: EDTA
• Cell Type: Mammalian Cells
• Concentration: 10 X
• Form: Liquid
• Without Additives: No Phenol Red
• Inorganic Salts: No Calcium, No Magnesium, No Sodium Phosphate
• pH: 6.9 to 7.4
• Purity or Quality Grade: Cell Culture Grade
• Remarks: Room Temperature Stable
• Source: Animal origin-free

• Content And Storage: Storage conditions: 15–30°C.
  Alternate storage conditions: 2–8°C or -5 to -20°C.
  Shipping conditions: Ambient.
  Shelf life: 24 months from date of manufacture.
• Classification: Animal Origin-free
• Culture Type: Adherent Cell Culture
• Product Type: Cell Culture Dissociation Reagent
• Green Features: Energy efficient, Sustainable packaging
• Osmolality: 270 - 320 mOsm/kg
• Product Line: Gibco™
• Quantity: 100 mL
• Shipping Condition: Room Temperature
• Tested For: In vitro Bioassay

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.