Thermo Scientific™ Tryptone Bile X-Glucuronide (TBX) Medium

Detect and enumerate Escherichia coli in food and animal feed samples with this selective, chromogenic, ready-to-use medium.

Product Code	Brand	Quantity	Fill Weight	Media Type
12972628	Thermo Scientific™ PO0727A	10/Pk.	19.5g ± 1.0.g	TBX Medium

Description

Detect and enumerate Escherichia coli in food samples with Thermo Scientific™ Typtone Bile X-Glucuronide (TBX) Medium. TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar^1,2, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity: an enzyme shown to be highly specific for Escherichia coli³. The released chromophore in TBX Medium is insoluble and accumulates within the cell, making colored target colonies easy to identify

Most Escherichia coli strains can be differentiated from other coliforms by the presence of the enzyme glucuronidase. However, approximately 3 to 4% of Escherichia coli are glucuronidase negative, notably Escherichia coli O157 strains⁴.
 

The chromogen in TBX Medium is 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-glucuronide) and is targeted by glucuronidase. Escherichia coli cells are able to absorb this complex intact and intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore is colored and builds up within the cells, causing Escherichia coli colonies to be colored blue/green.

 

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications

• Format: 90mm Monoplate
• Product Type: Agar
• Quantity: 10/Pk.

• Media Type: TBX Medium
• Packaging Type: Flow Wrapped - Single Layer

Safety and Handling

missing translation for 'shelfLife' : 12 Weeks