Invitrogen™ Tyramide Conjugates

Thermo Fisher Scientific offers a wide variety of bright Alexa Fluor™, Alexa Fluor™ Plus or Biotin XX tyramide conjugates. These tyramide conjugates are used in tyramide signal amplification (TSA), also called catalyzed reporter deposition (CARD).

Product Code	Brand	Conjugate	Excitation/Emission
15651902	Invitrogen™ B40952	Alexa Fluor™ 350	347/442 nm
15542414	Invitrogen™ B40953	Alexa Fluor™ 488	495/519 nm
15661902	Invitrogen™ B40954	Alexa Fluor™ 546	556/573 nm
15671902	Invitrogen™ B40955	Alexa Fluor™ 555	555/565 nm
15681902	Invitrogen™ B40956	Alexa Fluor™ 568	579/604 nm
15539135	Invitrogen™ B40957	Alexa Fluor™ 594	591/617 nm
15691902	Invitrogen™ B40958	Alexa Fluor™ 647	650/668 nm
17836578	Invitrogen™ B56131	Alexa Fluor™ Plus 750	750/790 nm
15595055	Invitrogen™ B40951	Biotin-XX

Description

Thermo Fisher Scientific offers a wide variety of bright Alexa Fluor™, Alexa Fluor™ Plus or Biotin XX tyramide conjugates. These tyramide conjugates are used in tyramide signal amplification (TSA), also called catalyzed reporter deposition (CARD). TSA is used to amplify signals up to 200 fold versus standard imaging methods. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification to detect low abundance targets. Up to seven-plex is possible in multiplexable fluorescent imaging.

These tyramide reagents are used in tyramide signal amplification (TSA), also called catalyzed reporter deposition (CARD). TSA is used to amplify signal up to 200-fold versus standard imaging methods. These reagents are provided stand-alone, but are also available as part of our SuperBoost™ tyramide signal amplification kits (available separately). They can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets. Up to seven-plex is possible in multiplex fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH).

Features include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO-3, secondary antibodies, and other SuperBoost kits
• Requires 10–100 times less primary antibody then standard ICC/IHC/ISH experiments

Tyramide reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. These reagents help sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. They are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a tyramide reagent can be imaged using any type of microscope, producing high-resolution multiplex images.

Dissolve the Alexa Fluor™ or Biotin-XX tyramide reagent in 150 μL of DMSO to prepare 100X stock. Tyramide may coat the sides of the vial so invert the vial several times to ensure all reagent is dissolved in solution.

Specifications

• Clonality: None
• Quantity: 1 vial, 150 slides
• For Use With (Application): Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ISH