Invitrogen™ Human ULK1, GST Tag Recombinant Protein

Product Code. 15431234

Brand: Invitrogen™ PV6430

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Validated in LanthaScreen™ Kinase Binding Assay Protocols available for simple use and optimized performance Multiple pack sizes available for convenient use Related Product:LanthaScreen™ Eu Kinase Binding Assay Protein Form: Recombinant, Fragment Assay Entry: Biochemical binding/kinetic The gene accession number for ULK1 is NP_003556.1.

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

Specifications

• Accession Number: O75385
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 10mM glutathione, 25% glycerol, 150mM NaCl and no preservative; pH 7.5
• Gene ID (Entrez): 8408
• Molecular Weight (g/mol): 96.1 kDa
• Name: Human ULK1, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: APG1; ATG 1; ATG1; ATG1 autophagy related 1 homolog; ATG1A; AU041434; Autophagy-related protein 1 homolog; C. elegans; EC:2.7.11.1; FLJ38455; hATG1; KIAA0722; mKIAA0722; Serine/threonine-protein kinase ULK1; serine/threonine-protein kinase Unc51.1; ULK 1; Ulk1; UNC 51; UNC51; unc-51 like autophagy activating kinase 1; unc-51 like kinase 1; Unc51.1; unc-51-like kinase 1
• Common Name: ULK1
• Gene Symbol: ULK1
• Protein Length: 1-649
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-649
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Serine/Threonine Kinase
• Target Entry: ULK1
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Fragment
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases