Thermo Scientific™ Varioskan™ LUX multimode microplate reader

Analyze a variety of samples with five measurement technologies, including absorbance (UV-Vis), fluorescence intensity, Luminescence, AlphaScreen / AlphaLISA and TRF.

Brand:  Thermo Scientific™ VLBL0TGD0

Product Code. 17173451

Description

Designed for bioscience researchers with a wide variety of needs, the Thermo Scientific™ Varioskan™ LUX multimode microplate reader comes equipped with a flexible range of measurement technologies including Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, and Time-Resolved Fluorescence. The instrument simplifies measurement setup with the automatic dynamic range selection, and its smart safety controls ease workflow and help avoid experimental errors. Varioskan LUX raises the bar for reliability and ease, with technology that works in researchers' best interest.

The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation.

The Varioskan LUX is a modular, upgradable instrument that provides excellent usability across a variety of applications.

• Up to five measurement technologies: Absorbance (UV-Vis), Fluorescence intensity (incl. FRET), Luminescence (direct and filtered), AlphaScreen/AlphaLISA and Time-resolved fluorescence (incl. TR-FRET/hTRF)
• Filter and monochromator optics
• Spectral scanning for assay optimization
• Five measurement modes including end point, kinetic, spectra, kinetic spectra and multipoint
• Integrated gas control module for setting CO2 and O2 concentrations for cell based assays
• Simultaneous dispensing and measurement for signal monitoring at the start of a reaction
• Automatic dynamic range selection, based on signal intensity of each well
• Smart safety controls designed to identify and alert the user of potential problems before they happen
• Automated instrument calibration and self-diagnostics for reliable performance
• Certified HTRF compatible reader by CisBio Bioassays
• Validated DLReady by Promega Corporation

Varioskan LUX is paired with the powerful and intuitive SkanIt Software, providing excellent usability and flexibility.

• License free—Each user can install the software on his/her computer
• Intuitive and flexible interface for multiple users and assays
• Innovative Virtual Pipetting Tool makes it easy to define samples to plate layout
• Measurement data is continuously saved to the database, so there’s no data lost due to unexpected interruptions such as power outages or accidental aborting
• Simplified data processing and export
  • Export data in a single mouse click, or have it emailed automatically at the end of the run
  • Several file formats for exporting results: *.xlsx, *.pdf, *.xml, and *.txt
• Shows real-time spectra and kinetic curves during the measurement
• Built-in calculations for data analysis (e.g. blank subtraction, average/SD/CV%, standard curve, dose response, quality control, classification, kinetic and spectral analysis, custom formula, etc.)
• Comprehensive result reports with traceability to runtime actions and settings
• Two editions available: Research Edition for life science researchers, and Drug Discovery Edition which offers features needed for the FDA 21 CFR Part 11 compliance.

The Varioskan LUX is modular and upgradeable, so you can build the system that best suits your needs. For more information, contact a sales representative.

Specifications

• Compatibility: Robot
• Incubator Temperature: 45°C
• Light Source: Xenon Flash Lamp
• Measurement Technology: Absorbance, Fluorescence Intensity, Luminescence, Time-resolved Fluorescence
• Product Line: Varioskan
• Read-out Range: 0 to 6 Abs
• Software Type: SkanIt Software
• Wavelength Selection: Double Monochromator (Absorbance and Spectral Scanning), Double Excitation and Emission Monochromator (Fluorescence Intensity), Filters (Time-Resolved Fluorescence)
• CO2 Concentration Range: 0.1 to 15%
• Depth (Metric): 58 cm
• Height (English): 20 in.
• Linearity: 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm
• No. of Dispensers: None
• Precision: SD <0.001 Abs or CV <0.5%, at 450 nm (0 to 3 Abs)
• Software Language Versions: English, German, French, Spanish, Portuguese, Italian, Japansese, Chinese and Russian
• Weight (English): 125 lb.
• Width (English): 21 in.

• Detector Type: Photomultiplier Tube (PMT)
• Interface: PC Software (Thermo Scientific SkanIt Software)
• Measurement Speed: 15 s for 96-well Plate, 45 s for 384-well Plate, 135 s for 1536-Well Plate (Minimum Times)
• Plate Type: 6 to 1536-well Plates (Absorbance 6 to 384-well Plates)
• Reading Options: Top and Bottom
• Shaking: Orbital
• Spectral Scanning Speed: <2.2 s/Well 400 to 500 nm, 1 Flash, 2 nm Step
• Accuracy (Photometric): 0.003 Abs or ±2% at 200 to 399 nm (0 to 2 Abs), 0.003 Abs or ±1%, at 400 to 1000 nm (0 to 3 Abs)
• Depth (English): 23 in.
• Dynamic Range: Fluorescence intensity: top reading > 6 decades; Luminescence: > 7 decades; Time-resolved fluorescence: > 6 decade
• Height (Metric): 51 cm
• Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra
• O2 Concentration Range: 1 to 21%
• Sensitivity: Fluorescence intensity (top reading): < 0.4 fmol fluorescein/well (black 384-well plate); Luminescence: < 7 amol ATP/well (white 384-well plate); Time-resolved fluorescence: < 1 amol/well (white low volume 384 well plate)
• Wavelength Range: Absorbance: 200 to 1000 nm, Fluorescence intensity: Excitation: 200 to 1000 nm, Emission: 270 to 840 nm, Luminescence: 360 to 670 nm, Time-resolved fluorescence: Excitation 334 nm, Emission 400 to 700 nm
• Weight (Metric): 57 kg
• Width (Metric): 53 cm