Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

Brand:  Thermo Scientific™ AB4105C

Product Code. 11852143

Description

The Thermo Scientific Verso 1-step RT-qPCR ROX Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA.

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.

Verso One-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.

• Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over
• RT and qPCR steps performed in single tube - convenience and reduced contamination risk
• Use of the entire RT reaction as template for qPCR - improved sensitivity
• Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg
• Verso RT enzyme has a wide working temperature range (42°: to 57°C) - improved success with GC-rich and other difficult templates
• Contains an inert blue dye for ease of pipetting during reaction setup

Specifications

• Content And Storage: Contains:
  •1 x 100 μL Verso Enzyme Mix
  •1 x 500 μL RT enhancer
  •1 x 5 mL 2X 1-Step qPCR SYBR ROX Mix
  • 1 x 100μL 1M MgCl₂.
  Sufficient for 400 x 25 μL reactions
  
  Store at -20°C
• Format: Tube
• PCR Method: 1-step RT-qPCR
• Product Line: Verso
• Quantity: 400 reactions
• For Use With (Application): Gene Expression, Pathogen Detection
• Sample Type: RNA

• Detection Method: SYBR
• GC-Rich PCR Performance: High
• For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
• Product Type: 1-Step RT-qPCR Kit
• Sufficient For: 400 Reactions
• No. of Reactions: 400 Reactions

For Research Use Only. Not for use in diagnostic procedures.