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Thermo Scientific™ Vivid™ Cyan Fluorescent Standard
Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid™ CYP450 Screening Kits.
Brand: Thermo Scientific™ P2877
Description
Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid™ CYP450 Screening Kits. Vivid™ Fluorescent Standards are included in the Vivid™ Screening Kits, but may also be purchased separately. They are supplied as a dried film.
More About Vivid™ CYP450 Screening Kits
Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent. Vivid™ Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
Applications
- High-throughput screening of enzyme-drug interactions
- Compound profiling for drug inhibition of cytochrome P450 isozymes
- Generation of predictive SAR models to guide compound acquisition
Specifications
Vivid™ Cyan Fluorescent St and ard, 1 Each, Powder, 0.1μM Concentration, 584.52 Molecular Weight, –20°C Storage Requirements, Used for Cell Viability, Proliferation and Function and Pharma and Biopharma and Protein Assays and Analysis a | |
0.1 μm | |
Cell viability, proliferation and function and pharma and biopharma and protein assays and analysis and target-based adme/tox assays, protein biology, cell analysis, proliferation and function, cell metabolism, drug discovery and development, target-based | |
Fluorescent Standard | |
• Vivid™ CYP450 Fluorogenic substrates • BACULOSOMES™ CYP450 reagents • Fluorescent standards • NADPH-regeneration system. Vivid™ CYP450 Fluorogenic substrates are also available separately. Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.) |
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Other Label(s) or Dye(s) |
Powder | |
Powder | |
Each | |
Vivid™ CYP450 Fluorogenic substrates, BACULOSOMES™ CYP450 reagents, fluorescent standards and an NADPH-regeneration system. Vivid™ CYP450 Fluorogenic substrates are also available separately. Store reaction buffers at 4°C or room tem | |
Wet Ice | |
VIVID™ |
For Research Use Only. Not for use in diagnostic procedures.