Thermo Scientific™ Vivid™ Red Fluorescent Standard

Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes.

Brand:  Thermo Scientific™ P2874

Product Code. 10072582

Description

Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid™ CYP450 Screening Kits. Vivid™ Fluorescent Standards are included in the Vivid™ Screening Kits, but may also be purchased separately. They are supplied as a dried film.

More About Vivid™ CYP450 Screening Kits

Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent. Vivid™ Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.

Applications

• High-throughput screening of enzyme-drug interactions
• Compound profiling for drug inhibition of cytochrome P450 isozymes
• Generation of predictive SAR models to guide compound acquisition

Specifications

• Description: Vivid™ Red Fluorescent Standard, Cell Viability, Proliferation and Function, Pharma and Biopharma, Protein Assays and Analysis, Target-Based ADME/Tox Assays, For Research Use Only, Not for Use in Diagnostic Procedures
• Quantity: Each
• Kit Contents: Vivid™ CYP450 Screening kits include Vivid™ CYP450 fluorogenic substrates, BACULOSOMES™ CYP450 reagents, fluorescent standards, and an NADPH-regeneration system. Vivid™ CYP450 fluorogenic substrates are also available separately
• Shipping Condition: Wet Ice
• Product Line: VIVID™

• For Use With (Application): Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target-based ADME/Tox assays
• Product Type: Fluorescent Standard
• Content And Storage: • Vivid™ CYP450 Fluorogenic substrates
  • BACULOSOMES™ CYP450 reagents
  • Fluorescent standards
  • NADPH-regeneration system.
  
  Vivid™ CYP450 Fluorogenic substrates are also available separately.
  
  Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
• Label or Dye: Other Label(s) or Dye(s)

For Research Use Only. Not for use in diagnostic procedures.