Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 20 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

Brand:  Thermo Scientific™ PV4532

Product Code. 10269952

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon a synthetic peptide substrate for NDR1.

Specifications

• Product Line: Z ́-LYTE™
• Assay Entry: Biochemical phospho-specific activity (no antibody)
• Excitation/Emission: 405/465, 535
• For Use With (Application): FRET
• No. of Reactions: 800 Reactions
• Readout: End Point
• Shipping Condition: Dry Ice
• Substrate Properties: Peptide-Based Substrate
• Target Enzyme: Serine/Threonine Kinases

• Assay: Serine/Threonine Kinase Assay
• Content And Storage: Z ́-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
• Format: 384-well Plate
• Label or Dye: Methylcoumarin
• Quantity: 800 x 20 μL
• References: EMBO J. vol. 17, pp. 5913-22 (1998)
• Source: Based on Synthetic Peptide Substrate for NDR1 (KKRNRRLSVA)
• Target Entry: PAK4, RPS6KA6, PAK7, PAK6, PAK2, PAK3
• Validated Kinases: PAK2, PAK3, PAK4, PAK6, PAK7, RPS6KA6

For Research Use Only. Not for use in diagnostic procedures.