Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 22 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-161.

Brand:  Thermo Scientific™ PV4572

Product Code. 10796323

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-161.

Specifications

• Description: Z'-LYTE™ Ser/Thr 22 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Serine/Threonine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Serine/Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis
• For Use With (Application): Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acute-lyte™ kinase assays, enzyme and protein activity assays, protein assays and, FRET
• Sample Volume: 800 x 20μL
• Assay Entry: Biochemical phospho-specific activity (no antibody)
• Product Type: Z'-LYTE™ Ser/Thr 22 Peptide Kinase Assay Kit
• Format: 384-well plate
• Label or Dye: Methylcoumarin
• Readout: End Point
• Shipping Condition: Dry Ice
• Substrate Properties: Peptide-Based Substrate
• Target Enzyme: Serine/Threonine Kinases

• Product Line: Z ́-LYTE™
• Includes: Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer
• Assay: Serine/Threonine Kinase Assay
• Content And Storage: –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
• Quantity: 800 x 20μL, 800 x 20 μL
• Excitation/Emission: 405/465, 535 nm
• No. of Reactions: 800 Reactions
• References: JBC v 277:27839-27849 (2002)
• Source: Based on CDK7 (GLAKSFGSPNRA)
• Target Entry: NEK7, NEK6
• Validated Kinases: NEK6, NEK7

For Research Use Only. Not for use in diagnostic procedures.