Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

Brand:  Thermo Scientific™ PV3177

Product Code. 12030176

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon glycogen synthase, containing Ser-7 and a consensus seq Hyd-X-R-X-X-S/T-X-X, where Hyd is bulky hydrophobic, F > L > V >> A, and X is any residue.

Specifications

• Description: Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide, 800 x 20μL Assays, 800 rxns, 405/465nm Excitation, 535nm Emission, Methylcoumarin Label, FRET Technique, Serine/Threonine Kinases Target Enzyme, Peptide-Based Substrate Properties, End Point Readout, Used For Pharma and Biopharma, Protein Assays and Analysis
• For Use With (Application): Pharma and biopharma, protein assays and analysis, target and lead identification and validation, FRET
• Sample Volume: 800 x 20μL
• Assay Entry: Biochemical phospho-specific activity (no antibody)
• Product Type: Z'-LYTE™ Kinase Assay Kit - Ser/Thr 4 Peptide
• Format: 384-well plate
• Label or Dye: Methylcoumarin
• Readout: End Point
• Shipping Condition: Dry Ice
• Substrate Properties: Peptide-Based Substrate
• Target Enzyme: Serine/Threonine Kinases

• Product Line: Z ́-LYTE™
• Includes: All necessary reagents for performing primary or secondary screens
• Assay: Serine/Threonine Kinase Assay
• Content And Storage: –80°C, Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
• Quantity: 800 x 20μL, 800 x 20 μL
• Excitation/Emission: 405/465, 535 nm
• No. of Reactions: 800 Reactions
• References: Biochem J. vol 296, pp. 843-9 (1993), J. Biol Chem, vol. 260, pp 14471-6 (1985)
• Source: Ser 7 Glycogen Synthase (KKLNRTLSVA) Based
• Target Entry: MAPK8, MAPKAPK2, MAPKAPK5, CAMK2D, MAPK9, STK22B, STK22D, CAMK2A, MAPK14, MAPK10, MAPKAPK3
• Validated Kinases: MAPKAPK2, MAPKAPK3, MAPKAPK5, STK22B, STK22D, CAMK2A, CAMK2D, MAPK8, MAPK9, MAPK10, MAPK14

For Research Use Only. Not for use in diagnostic procedures.