Thermo Scientific™ Z'-LYTE™ Kinase Assay Kit - Tyrosine 7 Peptide

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format.

Brand:  Thermo Scientific™ PV4305

Product Code. 10492902

Description

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon LAT, containing Tyr-200.

Specifications

• Description: Z'-LYTE™ Tyrosine 7 Peptide Kinase Assay Kit, 800 x 20μL, 800rxns, Liquid, Tyrosine Kinase Assay Type, Peptide-Based Substrate, 535nm Emission, 405/465nm Excitation, Methylcoumarin Label, End Point Readout, Threonine Kinases Target Enzyme, FRET Technique, Used for Pharma and Biopharma, Protein Assays and Analysis
• Formulation: Liquid
• Includes: Z'-LYTE™ Ser/Thr 11 peptide, Z'-LYTE™ Ser/Thr 11 phospho-peptide, 5X kinase buffer A, ATP, development reagent B, development buffer
• Assay: Tyrosine Kinase Assay
• Content And Storage: Z'-LYTE™ Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. See COA for specific storage instructions Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.
• Quantity: 800 x 20μL, 800 x 20 μL
• Excitation/Emission: 405/465, 535 nm
• No. of Reactions: 800 Reactions
• References: Biochem. J. 356 p 461-71 (2001)
• Source: Based on LAT, Residues 195 to 208, Centering Y-200 (ESIDDYVNVPESGES)
• Target Entry: ZAP70
• Validated Kinases: ZAP70

• Product Line: Z ́-LYTE™
• For Use With (Application): Pharma and biopharma, protein biology, drug discovery and development, target and lead identification and validation, kinase biology, biochemical kinase assays, z and acutelyte™ kinase assays, enzyme and protein activity assays, protein assays and analysis, kinase activity, FRET
• Sample Volume: 800 x 20μL
• Assay Entry: Biochemical phospho-specific activity (no antibody)
• Product Type: Z'-LYTE™ Tyrosine 7 Peptide Kinase Assay Kit
• Format: 384-well plate
• Label or Dye: Methylcoumarin
• Readout: End Point
• Shipping Condition: Dry Ice
• Substrate Properties: Peptide-Based Substrate
• Target Enzyme: Tyrosine Kinases

For Research Use Only. Not for use in diagnostic procedures.