Invitrogen™ Zenon™ Rabbit IgG Labeling Kits

Generate antibody (IgG) conjugates for immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry, and cell imaging with the Invitrogen Zenon™ Rabbit IgG Labeling Kits.

Product Code	Brand	Quantity	Excitation/Emission	Label or Dye
10758853	Invitrogen™ Z25300	50 Reactions	346/442 nm	Alexa Fluor 350
10247492	Invitrogen™ Z25302	50 Reactions	495/519 nm	Alexa Fluor 488
10626743	Invitrogen™ Z25303	50 Reactions	531/554 nm	Alexa Fluor 532
10339032	Invitrogen™ Z25304	50 Reactions	556/573 nm	Alexa Fluor 546
10565013	Invitrogen™ Z25305	50 Reactions	555/565 nm	Alexa Fluor 555
10013402	Invitrogen™ Z25306	50 Reactions	578/603 nm	Alexa Fluor 568
10387912	Invitrogen™ Z25307	50 Reactions	590/617 nm	Alexa Fluor 594
10114712	Invitrogen™ Z25308	50 Reactions	650/668 nm	Alexa Fluor 647
10553863	Invitrogen™ Z25312	50 Reactions	752/779 nm	Alexa Fluor 750
10398542	Invitrogen™ Z25313	50 Reactions	402/421 nm	Alexa Fluor 405
10347962	Invitrogen™ Z25351	25 Reactions	650/660 nm	APC (Allophycocyanin)
10134712	Invitrogen™ Z25352	25 Reactions		Biotin-XX
10357962	Invitrogen™ Z25354	25 Reactions		HRP (Horseradish Peroxidase)
10554053	Invitrogen™ Z25355	25 Reactions	496, 546, 565/578 nm	R-PE (R-Phycoerythrin)
10759043	Invitrogen™ Z25360	3 x 10 μL	495, 555, 650/519, 565, 668 nm	Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 647
10769043	Invitrogen™ Z25370	3 x 10 μL	346, 495, 590/442, 519, 617 nm	Alexa Fluor 350, Alexa Fluor 488, Alexa Fluor 594

Description

Generate antibody (IgG) conjugates for immunocytochemistry (ICC), immunohistochemistry (IHC), flow cytometry, and cell imaging with the Invitrogen Zenon™ Rabbit IgG Labeling Kits. Simplify your laboratory applications and reduce antibody cross-reactivity while also achieving efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions.

Achieve fast, versatile, and reliable fluorophore-, biotin-, or enzyme-labeled IgG primary antibodies with the Zenon™ rabbit IgG labeling kits. These kits utilize Alexa Fluor fluorophores, biotin, Pacific Blue, or enzymes such as R-phycoerythrin and allophycocyanin, which are attached to monovalent, affinity purified Fab fragments. The Fab fragments, in turn, are directed against and bind with the Fc portion of IgG primary antibodies. Only a small amount of starting material is required, and the method is optimized for efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Because the Zenon labeling method is based on immunoselectivity, it does not require the removal of exogenous proteins (such as serum) or amine-containing buffers from the target antibody, simplifying the process.

Zenon labeling technology greatly simplifies the use of multiple mouse-derived antibodies in the same staining protocol. Zenon tricolor labeling kits contain sufficient materials for 10 labeling reactions of each of three different fluorescent colors.

Important features of Zenon labeling technology:
• Labeled antibodies are typically ready to use in 10 minutes
• Requires only 1–20 μg primary antibody
• Simple—no purification required
• Flexible—choose from different fluorophores, biotin, HRP, alkaline phosphatase
• Multiplex with other mouse monoclonal antibodies simultaneously
• Can be used in a variety of applications including ICC, IHC, flow cytometry, and cell imaging.

Advantages of using Zenon antibody labeling kits include:

Cost savings
Zenon antibody labeling kits offer a cost-conscious and reproducible method of tagging as little as 0.4 μg in 2 μL of primary antibody, with minimal waste of expensive or difficult-to-obtain antibodies, or excessive washing steps that pose the risk of product loss.

Sensitivity
Label your primary antibodies without compromising their antigen binding affinity: Zenon dye- and enzyme-labeled Fab fragments, which are targeted to the Fc tail, are affinity purified during their preparation to ensure high affinity and selectivity for the Fc portion of the corresponding primary antibody. The procedure for chemical labeling of the Zenon Fab fragments protects their Fc-binding site, resulting in more active labeling reagents.

Speed
No purification procedure is required prior to using Zenon Fab fragments in your laboratory applications. Formation of the Fab-antibody complex occurs in fewer than five minutes, followed by a five-minute blocking step. During this time, almost all the primary antibody in the mixture is labeled with the labeled Fab fragments.

Simplicity
The Fab-antibody complexes display fluorescence or enzymatic activity that is similar in intensity to that of directly labeled primary antibodies. Varying the extent of the antibody labeling is as simple as changing the amount of added Zenon labeling reagent during the reaction. Once the labeling complexes are formed, they can used immediately, without need for antibody purification.

Reliability
The Zenon Fab-antibody complex is stable and allows subsequent or simultaneous labeling of different target cells and tissues with different complexes. After staining, an aldehyde-based fixing step may be used to prevent the transfer of different labels between different primary antibodies, preserving the initial staining pattern.

Customization
We offer custom antibody conjugation services that are efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.

Specifications

• Labeling Scale: < 1–20 μg
• Product Line: Zenon™
• Labeling Target: IgG
• Species: Rabbit

For Research Use Only. Not for use in diagnostic procedures.